Wpływ terapii fotodynamicznej (PDT) na biofilm Enterococcus faecalis w pierwotnych i wtórnych zakażeniach doświadczalnych kanału korzeniowego
Antimicrobial treatment of secondary infections
For antimicrobial treatment of the secondary endodontic infections, 70 specimens were randomly divided into three groups. In the first group (PDT group), 20 root canals were treated using PDT (PACT, Cumdente, Tübingen, Germany) and sampled according to treatment of the PDT group of the primary infections.
In the second group (NaOCl group), 20 root canals were irrigated with 10 mL of 3% NaOCl with a flow rate of 3–3.5 ml per minute. NaOCl was removed by rinsing the root canals with 2.5 mL of sterile Ringer’s solution.
In the third group (NaOCl-PDT group), 20 root canals were rinsed with 10 mL of 3% sodium hypochlorite (NaOCl) using a 30 gauge irrigation needle with a flow rate of 3–3.5 ml per minute. NaOCl was removed by irrigation with 2.5 mL of sterile Ringer’s solution and a sample was taken using 3 sterile ProTaper F2 paper points. Afterwards root canals were treated using PDT and sampled as described above.
In the control group, 10 root canals were rinsed with 10 mL of Ringer’s solution.
After antimicrobial treatment samples were taken from all specimens using 3 sterile ProTaper F2 paper points and transferred into a vial containing 750 μl of sterile Ringer’s solution to determine the survival fractions.
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