Reakcja ludzkich komórek struktur jamy ustnej na różne materiały endodontyczne – badanie in vitro
PDL cells
During the first days of cell culture, the PDL cells appeared as long, slender cells without an ordered arrangement. The cells were similar to fibroblasts. In addition to that, polygonal cells could occasionally be identified. In the further course of the cultivation a remarkably strong growth of the cultures took place so that after twenty days a confluent monolayer could be recognized in all cultures. An exceptional result was the organized spreading and the strictly parallel alignment of the cells (Figure 2a-e). The presence of Biodentine had impressive stimulatory effects. Only in this group beyond the forming of a confluent cell layer, the PDL cells matured in a second cell layer crossway to the first one (Figure 2b). In contrast the culture under the influence of the polymerized composite resin showed reduced cell density and growth. An increase of cells had occurred and they ordered themselves in a parallel arrangement, but no confluent monolayer was formed after 20 d (Figure 2d).
Cell count
Osteoblasts
One day after the application of the human osteoblasts onto the material samples the cell quantity was significantly decreased in all groups compared to the untreated control group (p < 0.05). In the following days it became obvious that the light cured composite resin samples had a negative impact on the osteoblasts. The number of cells dropped [...]
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