Wpływ terapii fotodynamicznej (PDT) na biofilm Enterococcus faecalis w pierwotnych i wtórnych zakażeniach doświadczalnych kanału korzeniowego
In the present study, a monospecies infection model with a clinical isolate of E. faecalis was used. E. faecalis has frequently been found in primary and most importantly secondary infections of root canal systems [32, 33].
E. faecalis has been shown to be very resistant against disinfecting agents and antibiotics [32]. E. faecalis can effectively be colonized, forms biofilm on root canal walls and invades dentinal tubules [34-36]. This monoinfection model was used to reproduce the same biofilm-like structure in each root canal of the specimens with a species that is known to be hard to eliminate by chemo-mechanical debridement [32]. However, root canal infections are often associated with multiple species [27, 37]. A biofilm with multiple species may differ in the content of the microorganisms in each specimen that might impair the effect of disinfection [27].
Samples from the root canals were taken using sterile ProTaper F2 paper points. Paper points only take a portion of bacteria from the root canal wall. This method of sampling will underestimate the CFU levels in the root canals before and after treatment and does not give the total E. faecalis counts for each specimen. A culture-negative sample does not mean a sterile root canal. For all samples ProTaper F2 paper points were used. These paper points have the same surface size and perfectly fit into the prepared root canal for sampling and this method has been used [...]
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