Odporność na przecieki bakteryjne czterech środków łączących używanych do cementowania koron
In preparation for the bacterial leakage test, the occlusal surfaces of the gold copings were reduced until the dentin was exposed using a fine high-speed diamond bur with light pressure and copious water-cooling. This was followed by sanding the occlusal surface on wet garnet paper of 400 and 600 gritm to remove gold flash that may have been caused by the diamond bur. Subsequently the entire root surface 1 mm below the margin of the copings was coated with two layers of nail polish and the samples were prepared for bacterial microleakage.
Bacterial leakage setup – For this experiment, a slight modification of the dual chamber test apparatus described by Imura et al (12) was used. The tip of 1.5 ml Eppendorf plastic tubes (upper chamber) was cut, and the samples were pushed (crown first) through the opening until approximately one-half of the crown protruded through the end. The junction between the sample and the tube was then sealed with two layers of cyanoacrylate (Cianon) and covered with sticky wax making sure the crown margin was located in the upper chamber. The tubes were placed into glass vials (lower chamber) containing 10 ml of sterile trypticase soy brotho (TSB) in such a way that the occlusal dentin/cement interface was submerged in the broth of the lower chamber (Figure). The junction between the tube and the glass vial was sealed with two layers of cyanoacrylate and finally covered with sticky wax. The [...]
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