Wpływ terapii fotodynamicznej (PDT) na biofilm Enterococcus faecalis w pierwotnych i wtórnych zakażeniach doświadczalnych kanału korzeniowego
Statistical analysis
A one-way analysis of variance (ANOVA) was applied to the data in order to assess the effect of different treatment techniques on the reduction of E. faecalis in root canals. The significance level was set to p < 0.05. All statistical tests were conducted using SSPS (15.0).
Results
Effect of antimicrobial treatments in primary infections
Figure 1 shows 72 hours old E. faecalis biofilm. Mean E. faecalis counts before antimicrobial treatments were 2,32 x 106 CFU/ml.
Treatment of root canals with PDT alone caused a reduction in bacterial load, resulting in a 92.7% kill of E. faecalis. Rinsing root canals with 3% sodium hypochlorite (NaOCl) achieved a reduction of 99.9% and the combination of NaOCl disinfection and PDT reduced bacterial viability by 99.9%. Irrigation with 3% sodium hypochlorite and the combination of NaOCl irrigation and PDT led to a significantly higher reduction of E. faecalis compared to PDT treatment alone (p < 0.0001) cases. After irrigation with 3% NaOCl, 80% of the root canals were culture negative canals. The additional treatment using PDT increased the number of culture negative canals to 90%. When root canals were treated with PDT alone, 1 out of 20 cases (5%) was culture negative (Table 1).
Effect of antimicrobial treatments [...]
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